10xGenomics, ‘Single Cell’ Vaccine Response Analysis “Cell Paper”

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Reporter Yoon-seok Seo, Biospectator

Chromium Single Cell Gene Expression and Chromium Single Cell ATAC analysis in PBMC cells administered with ‘influenza vaccine + immune booster’..Protective effect on dengue and Zika virus infection

On the 21st, 10x Genomics conducted a study on the epigenetic response of immune cells during influenza vaccination using its single cell analysis product in the international scientific journal ‘CELL (IF: 41.582). ‘ was published in

According to the paper, the Stanford University research team used 10xGenomics’ Chromium Single Cell Gene Expression and Chromium Single Cell ATAC (assay for transposase accessible chromatin) to identify single-cell epigenome of cells involved in the innate immune response before and after influenza vaccination. and transcriptome analysis.

As a result, epigenetic reprogramming of innate immune cells, which appears as a result of influenza vaccination, showed a result of providing a protective effect against dengue fever and Zika virus. Specifically, PBMCs (peripheral blood mononuclear cells) were collected from vaccinated people (four patients), influenza vaccine (H5N1) and ‘vaccine + AS03 adjuvant’ were administered, and after infection with Zika virus or dengue virus, single cells were administered. RNA sequencing and single-cell ATAC-sequencing were performed.

The research team confirmed the result that the viral titer against dengue fever and Zika virus significantly decreased in PBMCs administered with the vaccine + AS03, and confirmed the result that the AS03 adjuvant showed an epigenetic protective mechanism.

The Chromium Single Cell Gene Expression of 10xGenomics used in this study enables single-cell transcriptome 3′ gene expression profiling tens of thousands of cells and multiomic analysis, and combined gene expression in each single cell. , surface protein expression, cell heterogeneity through CRISPR editing, and discovery of new targets and biomarkers are possible.

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Chromium Single Cell ATAC is capable of analyzing the level of chromatin accessibility in single cells, providing results on cell type and status, and analyzing gene regulation mechanisms.

10xGenomics said, “Single cell analysis technology used in this paper is needed to integrate various analytical methods to clearly analyze the immune response after vaccination and to understand the complexity of biology.” We are delighted to be used in the design of an innovative vaccine that can benefit human health.”

Meanwhile, DAON BioSciences is the sole distributor of 10xGenomics products in Korea.

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