When the saline sample was obtained Try to filter out bacteria or other germs that may be contaminated. before trying to inoculate the cells The next day, began to see changes in cells that said The virus in the sample is probably not a germ. This morning, which is about 40 hours after entering the cell. What you see in the petri dish is Cells are clearly deformed. When the virus infects it, some protein spikes are produced on the cell surface.
The spike protein binds to the ACE2 receptor protein of the neighboring cell and fuses the cell. forming a large cell with a central nucleus called Syncytium, and if the fusion continues The cells will shrink into lumps and ready to come off as in the picture. It is a condition in which virus particles are released outside the cell and continue to destroy other normal cells. while the cells that fell out eventually died. I tried taking 10 microliters of cell culture, which is very little. Dropped onto the ATK as expected. My sister’s virus has grown beautifully.
Before inoculating the virus into cells, RNA was extracted for RT-PCR to roughly examine the strains. As expected, she had BA.2. Fortunately, she had a high fever for only 1-2 days and her overall condition was considerably better. But the bad news is that the mother who takes care of the younger sibling has a sore throat, hot eyes and a faint positive ATK this morning. The mother had been infected with Delta and received the Moderna vaccine under the skin a few weeks ago. Which should have a Hybrid immune system, but it doesn’t seem to be enough to prevent BA.2 infection from daughter It’s not as heavy as when it was attached to the Delta.
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Anan Jongkaewwattana
