Dr. Anant Biotech explains the phenomenon of 2 weak lines in ATK.

Dr. Anant Biotech explains the phenomenon of 2 weak lines in ATK.

December 5th Dr. Anant Chongkaewwattana, Researcher in virology, Biotechnology Post a message via Anan Jongkaewwattana’s Facebook with a picture stating that

This picture is beautiful and clearly explained. According to an article in the latest edition of the journal Nature reviewing microbiology, samples collected from nose, throat or saliva swabs can be seen to contain either viable viruses that are infectious, or viruses whose particles cannot continue to infect. with fragments of protein that have escaped from such particles, or fragments of RNA genetic material attached to the sample Genetic testing by qRT-PCR or RT-PCR is the process of breaking the virus to release its genetic material to’ w measure. This is the use of the detection signal as a representative of the virus present in the specimen.

But because the sample collected has RNA fragments in the first place Therefore, RT-PCR tests can detect viral RNA that is isolated from the actual virus or from the original contaminating RNA that does not come directly from’ r viral particle. which is exactly what many people call it “Infection” there

In the case of ATK tests, which detect viral nucleocapsid proteins, they come from two sources: virus particles directly or The protein fragments are linked together with the broken particles. But since proteins cannot be amplified like RNA amplification by RT-PCR, their measurements are less sensitive. A very positive ATK signal requires some protein. The denatured viral protein alone would not be sufficient to produce such a strong signal.

Make the ATK check that gets a clear mark. It means that the whole virus particles are still present in the sample In other words, the ATK is clearly positive, that is, the infectious virus is still present ….On on the other hand, the ATK that faints after being sick for one. while it may mean that there are Protein fragments that are released from the virus as well.

Detection of complete infectious particles continues This can be done by incubating the sample with the cells in the laboratory. These changes in those cells were then measured, such as expression of viral proteins, cell death, or increased viral load. This is to confirm that the object actually contains a viral infection. The researchers were able to compare the viral loads in such samples. By continuously diluting the sample to find the last point where the cell changes, for example, if it is diluted 1000 times, it is still possible to find cells that express viral proteins at 5 points, it can be roughly said that Samples contained 5000 infectious viral particles per sample volume tested.

PS: The attached picture is an example of SARS-CoV-2 infected cells stained with 1 antibody, ie infected cells.

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