Bongchim is an acupuncture that uses bee venom extracted from bees. It is known to have pain relief and anti-inflammatory properties, and is mainly used to treat musculoskeletal disorders such as back pain and arthritis, and is widely applied to trauma and skin whitening.
A research team led by Hyunseong Kim, a senior researcher at the Spine and Joint Research Institute at Jaseng Oriental Hospital, announced on the 18th that they have proven the mechanism of neuronal regeneration effect of the apamin component contained in Bongchim.
The research team collected neurons from the cerebral cortex of rats on the 17th day of embryonic day to check the neurotoxicity of the components that make up Bongchim, and then added bee venom, melittin, PLA2 (phospholipase 2), and apamine at various concentrations, respectively. Cell viability was compared by treatment over time.
As a result, bee venom and melittin showed significant toxicity at concentrations above 0.1 μg/mL, and PLA2 showed toxicity from 0.01 μg/mL. On the other hand, apamine had no negative effect on cell viability up to a concentration of 10 μg/mL. No toxicity was found in the additional 48-hour apamine treatment experiment.
Apamine was found to have an effect on nerve regeneration. After inflicting physical laceration damage on neurons in the cerebral cortex, the research team conducted an experiment to examine the effect of apamin on nerve regeneration by treating it with apamin. It was confirmed that the amount of regeneration of
In particular, it was confirmed that apamine increased the production of brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF), which induce nerve regeneration, indicating that the function of damaged nerve cells was improved.
In addition, this study also revealed that apamine promotes the expression of genes and proteins related to nerve regeneration. After apamine treatment on damaged cortical neurons, the expression of NF200 (Neurofilament 200-kDa), one of the cell regeneration-related genes, and GAP-43 (Growth Associated Protein-43), a protein that plays an important role in nerve regeneration, were significantly increased. was analyzed to be
Kim Hyun-seong, senior researcher at the Spine and Joint Research Center at Jaseng Oriental Hospital, who is the first author of the paper, said, “This study can be used as important basic data to determine the treatment direction for patients with spinal and neurological diseases in the future.” Through this, apamine could be a promising candidate for the treatment of related diseases.”
The results of this study were published in the August issue of ‘Toxins (IF=4.546)’, an SCI(E)-level international academic journal.